An alternative method for Staphylococcus aureus DNA isolation

Isolation and characterization of a DNA probe for Staphylococcus aureus subspecies aureus biovar.

The gene encoding for polynucleotide phosphorylase (pnp) of a new biovar of Staphylococcus aureus subsp. aureus (NBSA) has been isolated from a genomic library of strain M280(0). The coding region consisted of a 1094-bp HindIII-HindIII DNA fragment encoding for a protein of 277 amino acids with a calculated molecular mass of 29.5 kDa. The nucleotide sequence of the structural gene, contained a ...

tent: a rapid dna extraction method of staphylococcus aureus

no abstract.

Short Communication: DNA Extraction Using Liquid Nitrogen in Staphylococcus aureus

TENT: A Rapid DNA Extraction Method of Staphylococcus aureus

Recently different methods are introduced for DNA extraction from bacteria. Bacterial DNA can be extracted using a commercial kit and conventional (e.g., boiling, phenol-chloroform and detergent methods). In the manual methods, chemically solution such as EDTA, SDS, NaCl, Tris hydrochloride, phenol-chloroform, proteinase K and lysozyme can be proposed (1). The phenol-chloroform DNA extraction m...

Short Communication: DNA Extraction Using Liquid Nitrogen in Staphylococcus aureus